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The running back can only return to the field if he tests negative twice before Sunday's game against the Raiders, according NFL coronavirus protocols.
Barkley was absent from practice Wednesday, according to an NFL Media report.
Giants Running Backs Coach Burton Burns also recently tested positive for COVID-19, according to NBC Sports.
Barkley missed the past three games due to an ankle injury, according to game records.
Aaron Rodgers, another NFL player, also tested positive for COVID-19 on Wednesday, according to NFL Media.
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